Adenovirus IgG, 96 Wells
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Manufacturer: DRG International Inc.
Model: Adenovirus IgG, 96 wells
Product Code: EIA3446
Block Scientific, a leading laboratory equipment supplier offers the Adenovirus IgG, 96 wells, just one of the many reliable infectious disease kits that we make available to an extensive clientele. We deal in brand new as well as refurbished lab equipment.
The DRG Adenovirus IgG Enzyme Immunoassay Kit is reliable and provides materials for the qualitative and semiquantitative determination of IgG-class antibodies to Adenovirus in serum. This assay is intended for in vitro diagnostic use only.
Principle of the Test
Microtiter wells as a solid phase are coated with Adenovirus antigen.Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation Adenovirusspecific antibodies of positive specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgG antibodies are dispensed into the wells. During a second incubation this anti-IgG conjugate binds specifically to IgG antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Adenovirus-specific IgG antibody in the patient specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.
- Solid phase enzyme-linked immunosorbent assay (ELISA)
- Contents of the kit – sample diluent, negative control, positive control, microtiterwells, enzyme conjugate, stop solution, cut-off control, substrate solution, wash solution
If you have any questions about the Adenovirus IgG, 96 wells or other infectious disease kits, dial 631-589-1118 and speak to one of our customer service representatives or send an email to email@example.com.