Adenovirus IgA, 96 wells
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Manufacturer: DRG International Inc.
Model: Adenovirus IgA, 96 wells
Product Code: EIA3445
The Adenovirus IgA, 96 wells is just one of many infectious disease kits available with us for an affordable price. We are Block Scientific, a popular laboratory equipment supplier based in Bellport, New York but serving clients situated both within the U.S. as well as outside of it. We deal in both new and refurbished lab equipment.
DRG Adenovirus IgA Enzyme Immunoassay Kit
– Accurate Measurement
The DRG Adenovirus IgA Enzyme Immunoassay Kit provides materials for measurement of IgA-class antibodies to Adenovirus in serum.
- Solid phase enzyme-linked immunosorbent assay (ELISA)
- Contents of the Kit – Sample Diluent, Microtiterwells, Low Control, High Control, IgG-RF-Sorbent, Enzyme Conjugate, Calibrator, Substrate Solution, Enzyme Conjugate, Wash Solution, Step Solution
Principle of the Test
Microtiter wells as a solid phase are coated with Adenovirus antigen. Donor samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper-immune anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pretreatment avoids false negative or false positive results. Diluted specimens and ready-for-use controls are pipetted into these wells. During incubation Adenovirus-specific antibodies of positive specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgA antibodies are dispensed into the wells. During a second incubation this anti-IgA conjugate binds specifically to IgA antibodies resulting in the formation of enzyme-linked immune complexes.
After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Adenovirus-specific IgA antibody in the donor specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.
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